FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_TAM_Rep2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_TAM_Rep2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56131062
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1838310.3275031568082571No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1777170.31661079207801196No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1706520.3040241782704913TruSeq Adapter, Index 9 (100% over 50bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG860030.15321819494525152No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC818970.14590317211528975No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG800780.1426625421767363No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG679810.12111119508125465No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT675800.12039679562806063No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA675170.12028455830748401No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC662980.118112855231565No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC630640.1123513394419653No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT582290.10373757047390265No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG582250.10373044429481844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA195500.040.8537371
ATCGGAA206550.038.677532
CGTCTGA222200.036.66001516
TCACGAT219550.036.5776330
ACGTCTG223450.036.474515
CGATCAG220350.036.46502733
ACGATCA222100.036.17828832
GTCACGA224150.035.91407829
ATCTCGT224150.035.60103640
GATCTCG227700.035.24893639
CGTATGC231500.034.6385544
CACGATC238050.033.81012331
AGTCACG238800.033.80263528
TCGGAAG245400.032.804713
CGGAAGA252950.032.5977334
GCACACG273300.029.98238611
TCGTATG282000.028.56926243
CTCGTAT294350.027.71506742
GATCAGA304750.026.64051834
ATCAGAT315600.025.81545435
Produced by FastQC (version 0.11.2)