FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_TAM_Rep3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_TAM_Rep3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17116431
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC7002634.091174147227305TruSeq Adapter, Index 1 (100% over 50bp)
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC577900.33762879656395656No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA373260.21807116214823055No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT246470.1439961403168686No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA241080.14084711935566474No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT206150.12043982767201877No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC202730.11844174758160739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA739750.043.5281451
ATCGGAA744100.043.2789542
CGTCTGA748200.043.1217716
ACGTCTG748700.043.1164815
ACGATCT744050.042.9881237
ATCACGA746600.042.90425534
TCACGAT747550.042.80144535
TCGGAAG753600.042.7624443
GATCTCG749400.042.73663739
ATCTCGT748000.042.71943340
CGGAAGA761550.042.6379664
CACGATC751500.042.56999636
CGTATGC753650.042.515744
CTCGTAT754950.042.44875742
CATCACG756500.042.41004633
GCACACG764450.042.35763511
TCGTATG759950.042.1634943
ACACGTC778300.041.58126413
CACACGT783750.041.33141712
ACATCAC777650.041.31500232