Basic Statistics
Measure | Value |
---|---|
Filename | RNAseq_TAM_Rep3.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 17116431 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 700263 | 4.091174147227305 | TruSeq Adapter, Index 1 (100% over 50bp) |
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC | 57790 | 0.33762879656395656 | No Hit |
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA | 37326 | 0.21807116214823055 | No Hit |
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT | 24647 | 0.1439961403168686 | No Hit |
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA | 24108 | 0.14084711935566474 | No Hit |
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT | 20615 | 0.12043982767201877 | No Hit |
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC | 20273 | 0.11844174758160739 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 73975 | 0.0 | 43.528145 | 1 |
ATCGGAA | 74410 | 0.0 | 43.278954 | 2 |
CGTCTGA | 74820 | 0.0 | 43.12177 | 16 |
ACGTCTG | 74870 | 0.0 | 43.11648 | 15 |
ACGATCT | 74405 | 0.0 | 42.98812 | 37 |
ATCACGA | 74660 | 0.0 | 42.904255 | 34 |
TCACGAT | 74755 | 0.0 | 42.801445 | 35 |
TCGGAAG | 75360 | 0.0 | 42.762444 | 3 |
GATCTCG | 74940 | 0.0 | 42.736637 | 39 |
ATCTCGT | 74800 | 0.0 | 42.719433 | 40 |
CGGAAGA | 76155 | 0.0 | 42.637966 | 4 |
CACGATC | 75150 | 0.0 | 42.569996 | 36 |
CGTATGC | 75365 | 0.0 | 42.5157 | 44 |
CTCGTAT | 75495 | 0.0 | 42.448757 | 42 |
CATCACG | 75650 | 0.0 | 42.410046 | 33 |
GCACACG | 76445 | 0.0 | 42.357635 | 11 |
TCGTATG | 75995 | 0.0 | 42.16349 | 43 |
ACACGTC | 77830 | 0.0 | 41.581264 | 13 |
CACACGT | 78375 | 0.0 | 41.331417 | 12 |
ACATCAC | 77765 | 0.0 | 41.315002 | 32 |