FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_TAM_Rep4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_TAM_Rep4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39222971
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2853430.7274895111846577No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2466500.6288406862397037No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1268490.32340487414887564No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1234320.3146931424445129No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1180370.3009384475235188No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC1081860.27582306296991116No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG865890.22076094133715676No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG832120.21215119068874205No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA791410.2017720687196286No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC765260.1951050571870244No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG762210.19432745163542048No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG743340.1895164953210709No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC738290.1882289844897267TruSeq Adapter, Index 10 (100% over 50bp)
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC725430.18495029354099668No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG721270.1838896905591369No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA660810.16847525395258814No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC607630.154916872564294No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG554280.141315149227222No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT516420.1316626422817384No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG492970.1256840028767836No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA468050.11933058309121969No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG459350.11711249512434946No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT447890.11419073787143764No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT418970.10681750752639313No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA401110.10226405337831243No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA393890.10042329531845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG91450.036.5934139
GATCGGA95350.035.5715871
ATCGGAA104350.032.491212
ATCTCGT104600.032.09800340
CGTCTGA106650.031.92929616
TAGCTTA108200.031.15165534
CGTATGC109550.030.84523244
ACGTCTG111750.030.70830215
AGCTTAT120600.028.22285335
GCTTATC120000.028.21692836
TCGGAAG124600.027.4049723
CGGAAGA127050.027.3597154
CACTAGC139750.024.52880131
TCACTAG137800.024.49239530
GCACACG144000.023.93785711
TCGTATG141200.023.86894643
GTCACTA142900.023.66422829
CGTTATG24900.022.4434723
TCGTTAT25950.022.298422
CTCGTAT160800.021.80919842
Produced by FastQC (version 0.11.2)