RNAseq_WT_Rep1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	RNAseq_WT_Rep1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53521894
Sequences flagged as poor quality	0
Sequence length	50
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA	164268	0.30691738973213467	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	162890	0.304342742429855	TruSeq Adapter, Index 7 (100% over 50bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC	161922	0.30253413677774554	No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC	131446	0.2455929530445989	No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA	128564	0.24020824076218228	No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC	90093	0.16832924485071474	No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT	87324	0.16315566112066213	No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC	84703	0.15825859974237833	No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG	76856	0.14359730991582623	No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG	73227	0.1368169071146847	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG	66603	0.12444066347876255	No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG	61327	0.11458301531705885	No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT	60466	0.11297432785170121	No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT	60429	0.11290519726375901	No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT	55585	0.10385469542613719	No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC	54398	0.10163691142918074	No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT	54110	0.10109881387979282	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	18650	40.371017	1
ATCGGAA	19520	38.58242	2
CGTCTGA	20705	37.047726	16
ACGTCTG	20920	36.603752	15
ATCTCGT	20510	36.332535	40
TCGTATG	21485	34.661484	43
CGTATGC	22005	33.831577	44
TCGGAAG	22750	33.27762	3
CATCTCG	22645	33.198555	39
CGGAAGA	23900	32.429527	4
ACACGTC	24600	31.244026	13
CTCGTAT	24440	30.903362	42
GCACACG	25530	30.269588	11
TCTCGTA	24835	30.182056	41
CACACGT	26405	29.199879	12
CACGTCT	26960	28.476429	14
ACCAGAT	27390	27.777767	32
GATCATC	27755	27.32486	36
AGTCACC	29930	25.544027	28
AGATCAT	31850	24.046669	35