FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_WT_Rep3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_WT_Rep3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59675992
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC2933850.4916298668315392No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2829920.4742141529880224No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1879020.3148703418285866No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA1846820.30947453709692835No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1543020.25856629245476137No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1250860.20960858095161616No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT1115080.18685571242787216No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1079240.18084994716133082No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT853750.14306423259792647No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG737860.12364436271122228No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC685140.11480998924994829No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT668540.1120283010963605No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC656360.10998727930655933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG30300.023.3820193
GTGATTA118050.020.9947031
TCGTTAT36750.020.35592
CCCTACG294350.019.4175824
CGACTAT68200.019.38522317
CCTACGT305750.018.72254225
TACGTTC306350.018.7011327
CCGACTA71250.018.61702316
GACTACC308900.018.61527619
TACCCTA308500.018.54795622
ACTACCC313150.018.46800420
ACCCTAC310850.018.450223
CTACGTT315300.018.19809226
TAGGACG35550.017.51341625
TGACTAC333300.017.34485818
TATCCGA140150.017.3311638
CTCGCTA748300.017.278081
GCCTATA143750.017.18496110
TTATCCG141550.017.1444537
TTGACTA339400.017.04608317
Produced by FastQC (version 0.11.2)