RNAseq_WT_Rep4.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	RNAseq_WT_Rep4.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67593602
Sequences flagged as poor quality	0
Sequence length	50
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT	781952	1.1568432172027168	TruSeq Adapter, Index 27 (97% over 44bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC	596958	0.8831575509173191	No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA	296894	0.4392338789697877	No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC	211025	0.3121967076114689	No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT	181719	0.2688405331617037	No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC	161458	0.23886580271310293	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG	156424	0.23141835228724755	No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC	155647	0.23026883520721386	No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG	147904	0.21881360901583555	No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA	138401	0.2047545860923346	No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG	102513	0.15166080363641518	No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG	83301	0.12323799521735801	No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT	80343	0.1188618413914382	No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG	77978	0.11536298953264837	No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC	77332	0.11440727777756243	No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT	69205	0.10238395048099375	No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC	68820	0.10181436994584192	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	84690	42.833626	1
ATCGGAA	86110	42.14136	2
TATCTCG	86710	41.974953	41
CGTCTGA	88375	41.733368	16
ACGTCTG	88935	41.48042	15
ATCTCGT	88370	41.116177	42
TCGGAAG	90050	40.386883	3
CGGAAGA	92190	40.20309	4
GCACACG	94395	39.22545	11
CTCGTAT	94860	38.35937	44
TCTCGTA	95795	38.23872	43
ACACGTC	97250	38.091988	13
CACACGT	98720	37.513638	12
CCTTTAT	98685	37.136864	37
ACATTCC	100545	36.48506	32
CACGTCT	101800	36.38297	14
GTCACAT	102375	35.665462	29
AGCACAC	104955	35.44228	10
GAACTCC	103930	35.340286	21
CATTCCT	104380	35.230656	33