FastQCFastQC Report
Mon 11 Jan 2021
G119-M10_GTTTCG-_AC6PMVANXX_L004_001.R_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M10_GTTTCG-_AC6PMVANXX_L004_001.R_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22267175
Sequences flagged as poor quality0
Sequence length125
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG1173070.5268158174532692No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1056920.4746538346242844TruSeq Adapter, Index 21 (97% over 40bp)
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG1045250.469412936306469No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC852330.3827741956489766No Hit
TACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGAAGGACTTGGG672460.30199609963994084No Hit
ATCGGTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAAC648300.29114604793827686No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT572270.25700161785228703No Hit
GGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGC555300.2493805343515736No Hit
GGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACC540330.24265763393874615No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT537670.24146305043185765No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA481890.21641272411071455No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC468920.21058800678577322No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG448440.20139061196581964No Hit
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA435300.195489549078408No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA365000.16391841353921185No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT361950.16254868433018557No Hit
ATAGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCG350230.15728533143517306No Hit
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG326430.14659695268932857No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC326400.1465834799430103No Hit
CTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAA311400.13984710678386458No Hit
CGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAG290970.13067216654110816No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG289920.13020062041996797No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC273300.12273671895963453No Hit
GAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCC271160.12177566305559642No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG266480.11967391462994294No Hit
TGCCCCGGGCGTGGGGGGGGCGCGCGCCTCGTCCAGCCGCGGCGCGCGCC261610.11748683881094032No Hit
AAGGAACCATAACTGATTTAATGAGCCATTCGCAGTTTCACTGTACCGGC242240.1087879356047635No Hit
CCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTCTCCCCGGG238480.10709935139953765No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA233070.10466976614680577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)