FastQCFastQC Report
Mon 11 Jan 2021
G119-M12_GAGTGG-_AC6PMVANXX_L004_001.R_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M12_GAGTGG-_AC6PMVANXX_L004_001.R_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18364863
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG325440.17720796501449534Illumina Single End PCR Primer 1 (100% over 50bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG271000.14756440056209513No Hit
AGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCG199450.10860413170520249No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)