FastQCFastQC Report
Mon 11 Jan 2021
G119-M3_AGTCAA-_AC6PMVANXX_L004_001.R_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG119-M3_AGTCAA-_AC6PMVANXX_L004_001.R_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19068542
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2423081.2707211699772327Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)