FastQCFastQC Report
Mon 31 Oct 2016
G137-M11-Index-15_ATGTCA_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M11-Index-15_ATGTCA_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823097
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4935303.5703287041970406TruSeq Adapter, Index 15 (97% over 40bp)
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG222440.16091907623884866No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT188450.13632979642695123No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG172910.12508774263828143No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG167870.12144167113925339No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC155350.11238436654246149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT625250.042.7667714
GATCGGA639600.042.5354961
ACACGTC633400.042.24348413
CACACGT637400.041.997412
TCGGAAG650500.041.8433073
ATCGGAA654200.041.6245772
CGGAAGA653650.041.5715374
GCACACG646450.041.2759111
CTCGTAT666650.041.2286644
AGCACAC660050.040.48101410
CGTCTGA709200.040.44302716
ACGTCTG715100.040.36955315
AGAGCAC661650.040.3478938
GAGCACA661050.040.312079
TCTCGTA666200.039.753843
ACATGTC729050.039.28291332
ATCTCGT673600.039.2640142
AGACGTC26750.039.1711213
CATGTCA733600.039.04947333
GTCACAT737650.038.9063729