Basic Statistics
Measure | Value |
---|---|
Filename | G137-M11-Index-15_ATGTCA_BC9WPLANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13823097 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT | 493530 | 3.5703287041970406 | TruSeq Adapter, Index 15 (97% over 40bp) |
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG | 22244 | 0.16091907623884866 | No Hit |
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT | 18845 | 0.13632979642695123 | No Hit |
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG | 17291 | 0.12508774263828143 | No Hit |
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG | 16787 | 0.12144167113925339 | No Hit |
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC | 15535 | 0.11238436654246149 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGTCT | 62525 | 0.0 | 42.76677 | 14 |
GATCGGA | 63960 | 0.0 | 42.535496 | 1 |
ACACGTC | 63340 | 0.0 | 42.243484 | 13 |
CACACGT | 63740 | 0.0 | 41.9974 | 12 |
TCGGAAG | 65050 | 0.0 | 41.843307 | 3 |
ATCGGAA | 65420 | 0.0 | 41.624577 | 2 |
CGGAAGA | 65365 | 0.0 | 41.571537 | 4 |
GCACACG | 64645 | 0.0 | 41.27591 | 11 |
CTCGTAT | 66665 | 0.0 | 41.22866 | 44 |
AGCACAC | 66005 | 0.0 | 40.481014 | 10 |
CGTCTGA | 70920 | 0.0 | 40.443027 | 16 |
ACGTCTG | 71510 | 0.0 | 40.369553 | 15 |
AGAGCAC | 66165 | 0.0 | 40.347893 | 8 |
GAGCACA | 66105 | 0.0 | 40.31207 | 9 |
TCTCGTA | 66620 | 0.0 | 39.7538 | 43 |
ACATGTC | 72905 | 0.0 | 39.282913 | 32 |
ATCTCGT | 67360 | 0.0 | 39.26401 | 42 |
AGACGTC | 2675 | 0.0 | 39.17112 | 13 |
CATGTCA | 73360 | 0.0 | 39.049473 | 33 |
GTCACAT | 73765 | 0.0 | 38.90637 | 29 |