FastQCFastQC Report
Mon 31 Oct 2016
G137-M12-Index-16_CCGTCC_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M12-Index-16_CCGTCC_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13616783
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1377331.0114944183218606TruSeq Adapter, Index 16 (98% over 50bp)
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC571710.4198568780893402No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC454760.3339702189570033No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG426450.31317969890538755No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT349440.25662449052760844No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG344140.252732234919217No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC281960.20706799836642764No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC223540.1641650601320444No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG219470.16117610157994No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG197470.1450195688658621No Hit
GCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCG186820.1371983382565471No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC182710.13418000419041706No Hit
GTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGA181290.13313717344250842No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG178910.13138933035798545No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG177400.13028040470351918No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG168020.12339184666451687No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA166170.12203322914083303No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG163700.12021929114975248No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG162400.1192645869439206No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG158850.11665751007414893No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA154690.11360245661548693No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCC154040.113125104512571No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC149490.10978363979215941No Hit
CCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGC145690.10699296595972779No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTC137890.10126474072473651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT201800.038.17997442
CATCTCG199450.037.60190639
TCTCGTA201850.037.15741
CGTCCAT206300.036.8606435
ACCCGTC216150.036.39615632
ATCTCGT206400.036.34861440
CGTATGC217200.036.30650344
TCGTATG219050.035.94151343
CACCCGT219450.035.92705531
TCACCCG220250.035.84493330
ACGTCTG227200.035.2046915
CGTCTGA225650.035.1551716
ACACGTC209750.035.11319713
CACGTCT210650.035.02543614
GATCGGA216050.034.8509751
CACACGT213700.034.47511312
GTCCATC218350.034.3181336
GCACACG215100.034.20778711
ATCGGAA221900.033.8336222
CCCGTCC233300.033.72805833
Produced by FastQC (version 0.11.2)