FastQCFastQC Report
Mon 31 Oct 2016
G137-M13-Index-18_GTCCGC_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M13-Index-18_GTCCGC_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558632
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC480320.3824620388590095No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC348900.2778168832401491No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG319330.25427132509337No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT318010.2532202551997702No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG287580.22898990909200936No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC239750.19090455075043206No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG198030.1576843719921087No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG192770.15349601771912738No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC181240.14431508145154665No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG170170.13550042711658403No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC164210.13075468729396642No Hit
GCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCG156210.12438456672669443No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTC153090.12190021970545836No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG149350.11892218834025872No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC149100.11872312207253147No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC146700.11681208590234987No Hit
GTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAG141730.11285464849993215No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA140750.11207430873044134No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC140210.11164432559215048No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA134180.10684284721456923No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG129990.10350649656746054No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCC126700.10088678448416993No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG126420.1006638302643154No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG126100.10040902544162453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA11450.024.1992281
CGCACAT12050.021.21629137
AGTCACG13050.020.73949628
TCACGTC13200.020.50112730
ACGTCCG13000.020.4882232
GTCGGAT21800.019.9886021
GTCACGT13950.019.0934229
GCCCCGT19650.018.5600571
ACGTCTG16450.018.33577215
TCGGATT23350.018.2365952
CTCGTAT14950.018.09902244
CGTCTGA16250.018.02320716
GTCGGTA8050.017.945481
TTCCGCG4000.017.90864214
TCGCGAG2550.017.42960711
CCGCACA15750.017.31484236
CATCTCG14700.017.24775541
GTCGGTT10050.016.2593861
GCCGGAT8200.016.1731761
CTCGCGA2501.8189894E-1216.1038631
Produced by FastQC (version 0.11.2)