FastQCFastQC Report
Mon 31 Oct 2016
G137-M13-Index-18_GTCCGC_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M13-Index-18_GTCCGC_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12558632
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC217970.1735618975060341No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT187330.14916433573338242No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG184020.14652869834867363No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT180500.14372584529907398No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG176720.14071596333103797No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC176660.14066818742678341No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC161320.1284534812390394No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC153300.12206743537034927No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC152470.12140653536149479No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC147190.11720225578709528No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC146740.11684393650518624No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC144730.11524344371265915No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG136570.10874592073404173No Hit
CTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC129370.10301281222349695No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC126590.10079919532636995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG11400.021.56881915
TCGTGTA10900.021.52841414
GATCTCG12400.021.09899334
CGGTGGC52150.019.7594621
GATCGGA13500.019.2559971
AGAGCGT13350.018.3311868
AAGAGCG12800.017.8734137
GTAGATC15250.016.88085431
ATCTCGG15950.016.26859335
GGTGGCG66900.016.2275332
GTGGTCG16750.016.01424841
CGTCGTG17700.015.60640312
GGCCTTA39500.015.3357424
TAGATCT17650.015.18971332
TGTAGGG17000.015.11905617
GCCACGA9350.015.0285261
GCGCGTA3250.014.97892413
CCTTATA40950.014.8539046
GTCGTGT17000.014.44825413
GTGTAGG17600.014.3516716
Produced by FastQC (version 0.11.2)