FastQCFastQC Report
Mon 31 Oct 2016
G137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13583523
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG478250.35208097339696043No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC448420.33012054383829587No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG369070.27170418160296117No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT331330.24392052047175097No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG322180.23718441821020952No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG246860.18173488571411112No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG244450.17996067735888546No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC241380.1777005862175814No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC231800.1706479239590495No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG227820.16771790352178886No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC216300.15923704034660227No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA209610.15431195574226214No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC200100.14731082650649613No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC183080.13478094011398958No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG179790.1323588880439927No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC165200.12161793372750206No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG155960.11481557472240449No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA151800.11175304079803156No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA146920.10816045292520945No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC145990.10747579990846262No Hit
GTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGC140830.10367707994457698No Hit
CCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACG138390.10188078600816591No Hit
GGCCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGG136900.10078386880929197No Hit
GTCGGGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGC136530.10051147997467225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACG19250.034.0858835
AACGATC17600.032.799838
ACGATCT17650.032.70748539
CGTGAAA20300.032.00845333
ACGTGAA20600.031.54405832
GATCTCG18400.031.37556841
AGTCACG21200.031.25738528
GAAACGA20900.031.18896136
GTCACGT22050.029.76025429
TCACGTG22750.028.84455530
CACGTGA23300.028.16367531
CTCGTAT22800.027.00175344
ACGTCTG25000.025.96587415
GCCGGAT7600.025.5596731
CGTCTGA25900.025.14749716
GTGAAAC26400.024.77395834
GATCGGA26700.024.2218021
TCGTCGA5550.023.9736488
GTCGGTA8450.023.8296281
CGTCGAG5500.023.8002999
Produced by FastQC (version 0.11.2)