FastQCFastQC Report
Mon 31 Oct 2016
G137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M14-Index-19_GTGAAA_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13583523
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG280830.20674312547635837No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC194680.14332069817233717No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT175270.1290313271453952No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC166200.1223541197670148No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT162340.11951244165449566No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG147860.10885246780235143No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT147490.10858007896773171No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG147330.10846228920140968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG19800.028.8620115
TCGTGTA19450.028.68103814
GTGGTCG23950.025.89245641
AGAGCGT23050.025.2080278
GTAGATC26200.024.31661231
ATCTCGG26500.023.95092435
TCTCGGT26350.023.68344536
GATCTCG27050.023.62247734
GTCGTGT24750.023.51297613
TGTAGGG24250.023.29867617
GAGTGTA26400.023.17162727
CCTGCCA68600.023.1527181
GTAGGGA23050.022.89494718
TAGATCT28200.022.73778732
AGTGTAG27250.022.68481328
GGTCGCC26950.022.6154243
AAGAGCG25450.022.4774977
GTGTAGG25550.022.37397416
GATCGGA27050.022.3433781
TGCCAGT76700.021.1719533
Produced by FastQC (version 0.11.2)