FastQCFastQC Report
Mon 31 Oct 2016
G137-M15-Index-20_GTGGCC_BC9WPLANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M15-Index-20_GTGGCC_BC9WPLANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13149018
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC345460.26272684393617834No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC309020.23501374779470222No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT276660.21040354496434638TruSeq Adapter, Index 20 (97% over 44bp)
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT276530.21030467826570776No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG271250.20628916927484622No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG225280.17132838361009164No Hit
GGCGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGT169360.12880049293414914No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC169270.12873204675816857No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC166690.12676992304672485No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA162300.12343127068500477No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG162160.12332479885570162No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC158280.12037400815787155No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT153450.11670073004691302No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG153170.11648778638830672No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG144520.10990934836350517No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC140440.1068064550523849No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG139810.10632733182052075No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG138360.10522458787416672No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC138200.10510290578353457No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTC134040.10193917142709821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG39800.037.6879941
GATCGGA42650.037.598371
AGTCACG46100.035.36615828
ACGTGGC46450.034.911132
CTCGTAT45150.034.73176244
GTCACGT47200.034.6275129
TCACGTG47550.034.28299730
CGTCTGA47950.034.13854216
ACGTCTG49000.033.8535515
ACACGTC46250.033.52700813
CGTGGCC50200.032.34330733
TCGGAAG50150.032.1284143
CACACGT49050.031.80438812
CACGTCT48000.031.6794914
CACGTGG53800.030.30030831
GGCCTTA55000.029.20858236
GCCTTAT53150.029.1421437
ATCGGAA54750.029.1264172
CGGAAGA56250.028.7280274
ATCTCGT54400.028.08236342
Produced by FastQC (version 0.11.2)