FastQCFastQC Report
Mon 31 Oct 2016
G137-M15-Index-20_GTGGCC_BC9WPLANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG137-M15-Index-20_GTGGCC_BC9WPLANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13149018
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG243380.18509367011285557Illumina Single End PCR Primer 1 (100% over 50bp)
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC209730.15950240542677788No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG184240.1401169273629407No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC175190.13323428411155877No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC174460.1326791095730495No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT151330.1150884423460368No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC150090.1141454061436375No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC149860.11397048813835377No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC144190.10965837905157633No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC139220.10587862911131463No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC132840.10102655574735694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG42400.036.36337734
TCGTGTA39450.036.19602614
CGTGTAG40150.035.53022815
AGAGCGT41350.034.6304638
GATCGGA42400.034.1307531
ATCTCGG45800.033.66391435
GTAGATC46250.033.58291231
AAGAGCG41850.033.568867
TAGATCT47550.032.74476632
GTCGTGT44950.032.7416113
GTGGTCG46750.032.45067241
TGTAGGG44300.032.31017717
TCTCGGT48900.031.79642736
GAGTGTA49250.031.46548727
GTAGGGA43100.031.11129418
GTGTAGG46650.030.92124216
TCGGTGG51050.030.46821838
ATCGGAA48150.029.6865632
CGTCGTG54450.029.38178612
AGTGTAG52750.029.29623828
Produced by FastQC (version 0.11.2)