FastQCFastQC Report
Mon 31 Oct 2016
G138-M10-Index-1_ATCACG_BC9WPLANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M10-Index-1_ATCACG_BC9WPLANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16446845
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1730731.0523173289466763TruSeq Adapter, Index 1 (100% over 50bp)
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT264880.16105216532410926No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC230360.14006333737564863No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG185020.1124957400644318No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT173480.105479196769958No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG170920.10392266723496209No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC170540.10369161988211112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA211200.042.1940771
GATCTCG193250.041.2258739
TCACGAT200800.041.1356835
ACGTCTG218050.041.0958915
CGTCTGA218150.041.01565616
CTCGTAT205550.040.9268742
ACGATCT195250.040.7613137
CACGATC195500.040.7428936
TCGGAAG221050.040.4846653
ACACGTC223900.040.04350313
CGGAAGA223750.039.930084
CACACGT228100.039.39509212
CACGTCT228450.039.2918614
TCGTATG222500.039.25035543
CGATCTC204650.038.8872838
ACATCAC230200.038.45076832
CGTATGC228550.038.2017444
GAACTCC235650.038.0715421
GTCACAT235750.037.64921629
CAGTCAC240300.037.0258327