Basic Statistics
Measure | Value |
---|---|
Filename | G138-M10-Index-1_ATCACG_BC9WPLANXX_L002_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16446845 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 173073 | 1.0523173289466763 | TruSeq Adapter, Index 1 (100% over 50bp) |
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT | 26488 | 0.16105216532410926 | No Hit |
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC | 23036 | 0.14006333737564863 | No Hit |
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG | 18502 | 0.1124957400644318 | No Hit |
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT | 17348 | 0.105479196769958 | No Hit |
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG | 17092 | 0.10392266723496209 | No Hit |
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC | 17054 | 0.10369161988211112 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 21120 | 0.0 | 42.194077 | 1 |
GATCTCG | 19325 | 0.0 | 41.22587 | 39 |
TCACGAT | 20080 | 0.0 | 41.13568 | 35 |
ACGTCTG | 21805 | 0.0 | 41.09589 | 15 |
CGTCTGA | 21815 | 0.0 | 41.015656 | 16 |
CTCGTAT | 20555 | 0.0 | 40.92687 | 42 |
ACGATCT | 19525 | 0.0 | 40.76131 | 37 |
CACGATC | 19550 | 0.0 | 40.74289 | 36 |
TCGGAAG | 22105 | 0.0 | 40.484665 | 3 |
ACACGTC | 22390 | 0.0 | 40.043503 | 13 |
CGGAAGA | 22375 | 0.0 | 39.93008 | 4 |
CACACGT | 22810 | 0.0 | 39.395092 | 12 |
CACGTCT | 22845 | 0.0 | 39.29186 | 14 |
TCGTATG | 22250 | 0.0 | 39.250355 | 43 |
CGATCTC | 20465 | 0.0 | 38.88728 | 38 |
ACATCAC | 23020 | 0.0 | 38.450768 | 32 |
CGTATGC | 22855 | 0.0 | 38.20174 | 44 |
GAACTCC | 23565 | 0.0 | 38.07154 | 21 |
GTCACAT | 23575 | 0.0 | 37.649216 | 29 |
CAGTCAC | 24030 | 0.0 | 37.02583 | 27 |