Basic Statistics
Measure | Value |
---|---|
Filename | G138-M11-Index-2_CGATGT_BC9WPLANXX_L002_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16919179 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 67988 | 0.4018398292257561 | TruSeq Adapter, Index 2 (100% over 50bp) |
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC | 31781 | 0.18784008372983108 | No Hit |
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG | 30979 | 0.18309990100583484 | No Hit |
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT | 26195 | 0.1548242973255381 | No Hit |
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC | 23594 | 0.139451210960059 | No Hit |
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC | 19773 | 0.1168673728199223 | No Hit |
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT | 19715 | 0.11652456658801234 | No Hit |
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG | 19483 | 0.11515334166037253 | No Hit |
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG | 17046 | 0.1007495694678802 | No Hit |
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA | 17026 | 0.10063136042239403 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATCTCG | 7360 | 0.0 | 41.035538 | 39 |
GATCGGA | 8450 | 0.0 | 39.87254 | 1 |
CGATGTA | 8020 | 0.0 | 39.606144 | 34 |
TCACCGA | 8120 | 0.0 | 39.47439 | 30 |
CACCGAT | 8140 | 0.0 | 39.296432 | 31 |
GTCACCG | 8340 | 0.0 | 39.118107 | 29 |
CGTCTGA | 8800 | 0.0 | 38.763233 | 16 |
ACGTCTG | 8985 | 0.0 | 38.088493 | 15 |
ACCGATG | 8515 | 0.0 | 37.564598 | 32 |
CCGATGT | 8575 | 0.0 | 37.223793 | 33 |
CTCGTAT | 8530 | 0.0 | 36.872696 | 42 |
ACACGTC | 9590 | 0.0 | 35.906284 | 13 |
TCGGAAG | 9600 | 0.0 | 35.247818 | 3 |
TCGTATG | 9105 | 0.0 | 35.026566 | 43 |
GTATCTC | 8860 | 0.0 | 34.262054 | 38 |
CGGAAGA | 9930 | 0.0 | 34.181053 | 4 |
CACACGT | 10170 | 0.0 | 33.86084 | 12 |
CACGTCT | 10160 | 0.0 | 33.853096 | 14 |
GATGTAT | 9025 | 0.0 | 33.833267 | 35 |
TCTCGTA | 9520 | 0.0 | 31.8164 | 41 |