FastQCFastQC Report
Mon 31 Oct 2016
G138-M13-Index-4_TGACCA_BC9WPLANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M13-Index-4_TGACCA_BC9WPLANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14295611
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC808850.5658030286358519TruSeq Adapter, Index 4 (100% over 50bp)
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG340430.23813602650491816No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC291140.20365691260065766No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC246060.17212275851658249No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG226640.15853816951230695No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG225850.15798555234889927No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT204780.14324676294003802No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC199550.13958829741519968No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG196380.13737083360760166No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC189910.13284496899083223No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA178740.1250313820094853No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC167130.11691000825358216No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCC157970.11050244721963966No Hit
GCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAACACCG154600.10814508033269793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA112450.037.7367941
CGTCTGA113900.037.72458616
CTCGTAT107050.037.59292642
ACGTCTG114600.037.5526615
ATCTCGT103350.037.1760640
ACCAATC109500.035.37056436
ATCGGAA120000.035.339192
AATCTCG109400.035.22068839
TCTCGTA110150.035.0994341
CGGAAGA122150.034.783494
ACACGTC125000.034.63421613
ACTGACC122150.034.46178432
GCACACG125450.034.30213511
GACCAAT116700.034.26277535
TCGGAAG124850.034.0887643
CCAATCT113850.033.98052637
CACGTCT128600.033.6226914
CACACGT129350.033.4198812
TCGTATG128950.032.46899843
CTGACCA134000.031.5120533
Produced by FastQC (version 0.11.2)