FastQCFastQC Report
Mon 31 Oct 2016
G138-M14-Index-5_ACAGTG_BC9WPLANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M14-Index-5_ACAGTG_BC9WPLANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20262309
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC882370.4354735681900814No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG814770.4021111315595868No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG568660.2806491599748084No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG536320.26468849132643274No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT520790.25702401439046263No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC516230.2547735304994115No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC444980.21960971970173782No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC426370.21042517908497002No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG409680.20218821063285533No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTC375270.18520594074446303No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC371380.1832861200567023No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG371330.18326144369824782No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC365020.18014728726128892No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG342380.16897383215308778No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA341260.16842108172370682No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG340210.16790287819616215No Hit
GCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCG318130.1570057983026515No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT318010.15694657504236068No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA317690.15678864634825182No Hit
GTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAG309070.15253444215069467No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC293380.14479100086767013No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC288540.14240232936927377No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG288450.14235791192405564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC271710.13409626711348643TruSeq Adapter, Index 5 (100% over 50bp)
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG270110.13330662364294218No Hit
GTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGC269570.13304011897163348No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC259850.12824303488807717No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG239210.11805663411805635No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGG236870.11690178054238537No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA228080.1125636767260829No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCC227420.1122379487944834No Hit
GGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGG225750.11141375842210284No Hit
GTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGA221190.10916327453105172No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC219830.10849207758108911No Hit
CGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCC218450.1078110100877447No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC217540.10736190036387265No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG217300.10724345384329102No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG215640.10642419874260134No Hit
GTCGGATTCCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGG214140.10568390798896612No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG211550.10440567262102261No Hit
GTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGG209540.10341368301115139No Hit
GCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGAC209530.10340874773946049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG40900.031.79528639
GATCGGA50450.031.2007371
ACGTCTG52650.029.94124815
CTCGTAT47100.029.52056342
CGTCTGA53400.029.43756516
AGTGATC48400.027.14198536
CGGAAGA61600.025.6507824
TCGGAAG62050.025.5395833
ACACGTC64250.024.77136413
TCTCGTA54950.024.02465641
CACACGT69200.023.09676212
GTCACAC64650.022.93872529
CACGTCT70300.022.72461914
TCCAGTC72800.021.55216625
GTGATCT60950.021.48119237
GAACTCC73700.021.2744321
ATCGGAA74500.021.1539542
GTCGGAT47550.020.5390821
GCACACG78000.020.519911
CAGTCAC74750.020.13809827
Produced by FastQC (version 0.11.2)