FastQCFastQC Report
Mon 31 Oct 2016
G138-M14-Index-5_ACAGTG_BC9WPLANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M14-Index-5_ACAGTG_BC9WPLANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20262309
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC543950.26845410362659067No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT387470.19122697220736296No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC361580.17844955379961877No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC319170.15751906655850526No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC311410.15368929572636564No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT301690.14889221164280933No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC271360.13392353260430487No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG266760.13165330762649013No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG262940.12976803384056576No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC262670.12963478150491142No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC260810.1287168209704037No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC259060.12785314842449597No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGG258030.12734481544033308No Hit
GCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCC255170.12593332773673524No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG252790.12475873307430066No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG234030.11550016338216931No Hit
GGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGG233830.11540145794835129No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC233210.1150954711035154No Hit
CTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC231250.11412815785209868No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC222280.10970121914535998No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA217070.10712994259440027No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG213730.10548156184963914Illumina Single End PCR Primer 1 (100% over 50bp)
GCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGG211650.10445502533793162No Hit
GCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCCGTC206730.10202687166600806No Hit
CCCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGC203940.10064993086424652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG40400.032.8363215
GATCTCG37100.032.66740834
TCGTGTA40750.032.2228814
ATCTCGG40150.030.07662835
AGAGCGT45100.029.1177868
GATCGGA48200.028.9714161
AAGAGCG45450.028.2638247
GTAGATC44100.027.83440431
GTGGTCG42800.027.55474941
GTCGTGT49650.027.45874413
CGTCGTG50000.027.26074412
TAGATCT46400.026.21420132
TGTAGGG53050.025.96555517
GTAGGGA50750.025.92399418
TCTCGGT47500.025.74808936
GTGTAGG53900.025.63665216
GAGTGTA49800.025.19193627
ATCGGAA56700.024.47112
TCGGTGG53800.023.55257238
AGTGTAG53150.023.47865928
Produced by FastQC (version 0.11.2)