FastQCFastQC Report
Mon 31 Oct 2016
G138-M15-Index-6_GCCAAT_BC9WPLANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M15-Index-6_GCCAAT_BC9WPLANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16539542
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC694220.4197335089448063No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC464850.2810537317176014No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG375170.22683215774656879No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG374550.22645729851527935No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT345070.2086333466791281No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG337660.20415317425355553No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG240040.14513098367536417No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC231500.13996760007018333No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG231380.1398950466705789No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG229430.13871605392700717No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC227070.13728917040145366No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC221250.13377033052063958No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC216680.1310072552190381No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCC208320.1259527017132639No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT207760.12561411918177662No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC201740.12197435696828848No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG198330.11991263119619637No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA178070.10766319889631767No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCC177650.10740926199770225No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC176660.10681069645096582No Hit
GCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGAC174820.10569821099036479No Hit
GTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAG172550.1043257425145146No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA171130.10346719395252903No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC170710.10321325705391357No Hit
GCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCG169790.10265701432361307No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG168920.10213100217648108No Hit
GTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTC166150.10045622786894583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG8450.021.83575639
GCCCCGT30500.018.5043411
GTCGGAT19800.017.3697241
CGCCAAT12300.016.07491733
GTCACGC12550.015.23041929
TCGGATT22650.014.7919212
TAGATCG8850.014.65547722
GTCCAGA257250.014.6460391
GTCGGCT132950.014.5426911
GAGTCGC248550.014.3692926
TCACGCC14000.014.28066330
CGGATTC23000.013.9915233
GCGGGTA27800.013.9572981
AGAGTCG254750.013.9247785
AGTCGCC259500.013.9089617
TCGGCTG135400.013.820962
GTCGGTA8500.013.7464471
GCCGGTA13800.013.7389291
GTCGGGT65750.013.3115391
AGTCACG16050.013.00447328
Produced by FastQC (version 0.11.2)