FastQCFastQC Report
Mon 31 Oct 2016
G138-M15-Index-6_GCCAAT_BC9WPLANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG138-M15-Index-6_GCCAAT_BC9WPLANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16539542
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG370710.22413558972793807No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC362230.2190084828225594No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC325850.19701271050915434No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC321290.1942556813241866No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC305880.18493861559165303No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT287250.17367470030306764No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC261080.15785201307267155No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC257740.15583261011701532No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC250280.1513222071082742No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC245800.14861354685637607No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT234990.14207769477534504No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA211950.12814744205129744No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC210960.127548876504561No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG193350.11690166511261314No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG191560.11581941023518062No Hit
CGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGC175960.10638746828660674No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC173050.10462804834619967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC81600.022.997621
TGGCGCA110400.017.5690274
CGTGTAG11750.017.22669215
GGTGGCG119150.016.7027112
GCGCACG123200.015.8535076
TCGTGTA13000.015.39614214
GATCTCG12050.015.15083134
GGGCGAT11950.014.7453651
GGCCTTA69450.014.7241044
CGCACGC131500.014.6878287
GTGGCGC136000.014.6494643
GGCGATC12300.014.4814582
GCGATCT12950.014.433833
ATCTCGG12650.014.43243335
ATCGTCG9350.014.35886324
CCTTATA69650.014.3054066
GCCTTAT71650.014.1187985
CGTCGGA9800.013.69948726
CACGCCT144450.013.4877469
ACGCCTG147250.013.37907110
Produced by FastQC (version 0.11.2)