FastQCFastQC Report
Mon 31 Oct 2016
G140-M14-Index-7_CAGATC_BC9WPLANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M14-Index-7_CAGATC_BC9WPLANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20684136
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1123850.5433391078070653TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT167700.042.56719640
CATCTCG171000.041.69046839
CGTATGC205050.039.3318344
TCGTATG206700.038.93966343
TCTCGTA190950.038.70480341
CGTCTGA214950.036.79297616
GATCATC199000.035.8749336
CTCGTAT221400.035.5062742
AGATCAT214900.034.13794735
GATCGGA230000.034.0306971
ATCGGAA230300.033.984462
TCGGAAG233750.033.4717673
GAACTCC240500.032.94629321
ACACGTC249600.032.9232313
CAGATCA239600.032.84486834
ACGTCTG251250.032.56557515
CGGAAGA241750.032.501664
ACCAGAT255300.031.85438732
CACGTCT258450.031.77295314
GCACACG264400.031.02089911
Produced by FastQC (version 0.11.2)