FastQCFastQC Report
Mon 31 Oct 2016
G140-M14-Index-7_CAGATC_BC9WPLANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M14-Index-7_CAGATC_BC9WPLANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20684136
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC147800.038.626442
GTCGCCG159450.036.40883344
ATCTCGG170150.036.14927335
GATCTCG173750.035.4095634
GCGTCGT175400.035.0704111
AGCGTCG170850.034.6675110
GAGCGTC171500.034.4196979
AGAGCGT174100.033.920328
TCGTGTA185250.033.54730614
AAGAGCG174250.033.3482067
CGTCGTG192900.033.3029512
CGTGTAG190550.033.08593815
CTCGGTG189500.032.60576237
GTGGTCG182450.032.2293641
TCGGTGG201250.031.89539338
ATCGGAA197050.031.8946442
GATCGGA202900.031.6742151
TCTCGGT195950.031.65597236
GTAGATC195650.031.6248331
TCGGAAG201400.031.5731643
Produced by FastQC (version 0.11.2)