FastQCFastQC Report
Mon 31 Oct 2016
G140-M15-Index-8_ACTTGA_BC9WPLANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG140-M15-Index-8_ACTTGA_BC9WPLANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23981746
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC155650.039.00216742
GCGTCGT166900.038.4269111
GAGCGTC166550.038.0925989
AGCGTCG167250.037.94956210
AGAGCGT170450.037.740038
AAGAGCG170150.037.444457
GATCGGA181800.037.2833331
ATCGGAA180900.036.957992
GTCGCCG166500.036.85170744
ATCTCGG177900.036.6327435
GATCTCG179650.036.45922534
CGTCGTG187250.035.65501812
TCGTGTA184800.035.5711114
TCGGAAG190300.035.508373
CGTGTAG189250.035.14415715
CGGAAGA184100.035.009964
CTCGGTG197650.033.3900437
TCGGTGG207100.033.03539738
GTAGATC202350.032.5816331
TCTCGGT205900.032.16868636
Produced by FastQC (version 0.11.2)