FastQCFastQC Report
Mon 31 Oct 2016
G141-M13-Index-12_CTTGTA_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M13-Index-12_CTTGTA_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12606655
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC400070.3173482577257805TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC62850.037.0074644
AATCTCG55600.036.529939
TCTCGTA56100.036.24263441
ATCTCGT57800.035.10230340
TCGTATG66150.035.0986643
CTCGTAT65100.033.61547542
CGTCTGA75700.030.42960216
ACACGTC76650.030.25199313
ACGTCTG77500.029.78766815
GTAATCT70800.028.80955137
CACGTCT83200.027.9795514
GATCGGA84950.027.8703351
ATCGGAA85600.027.7591132
CACACGT84900.027.31281712
GCACACG85200.027.12888111
TCGGAAG88800.027.0015033
TAATCTC79450.025.88927338
CCTTGTA91600.025.79179433
CGGAAGA94250.025.4161324
TGTAATC82000.025.05806736
Produced by FastQC (version 0.11.2)