FastQCFastQC Report
Mon 31 Oct 2016
G141-M13-Index-12_CTTGTA_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M13-Index-12_CTTGTA_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12606655
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG300740.23855654017659722Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC45050.036.61057742
ATCTCGG53300.033.42896335
GATCTCG57250.031.70124834
TCGTGTA59800.030.28892114
CGTCGTG63700.029.86080612
CGTGTAG63000.029.34895315
AGAGCGT68750.027.3954148
GTAGATC67150.027.29935531
CTCGGTG66850.027.11857837
GAGCGTC71850.026.3039349
AGCGTCG71750.026.26358210
TCGGTGG72000.026.10541538
GATCGGA76800.026.1040941
TCTCGGT70050.026.03306636
ATCGGAA75700.026.0187912
AAGAGCG73200.025.8263117
TAGATCT73450.024.95106732
GTGGTCG68750.024.72451841
TCGGAAG82400.024.4358673
GAGTGTA75500.024.41374427
Produced by FastQC (version 0.11.2)