FastQCFastQC Report
Mon 31 Oct 2016
G141-M14-Index-13_AGTCAA_BC9WPLANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M14-Index-13_AGTCAA_BC9WPLANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13547832
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1021610.7540763717766799TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA137800.040.24550243
AATCTCG138550.040.01907741
ATCTCGT139450.039.75301742
CTCGTAT162400.038.30279544
ACACGTC181250.037.71116613
CGTCTGA181550.037.55095316
ACGTCTG182800.037.26733815
CACGTCT187900.036.36701214
CACACGT192050.035.643412
AGTCAAC198500.035.173634
GCACACG195300.035.08876411
AACAATC163650.034.09172438
GTCAACA205950.033.85498435
TATCGTA15050.033.70717643
GATCGGA207850.033.630881
ATCGGAA210950.033.145912
TCGGAAG213700.032.8304523
CAACAAT187450.032.82459637
CAATCTC170700.032.68323540
ACAATCT172300.032.35478639
Produced by FastQC (version 0.11.2)