FastQCFastQC Report
Mon 31 Oct 2016
G141-M15-Index-14_AGTTCC_BC9WPLANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG141-M15-Index-14_AGTTCC_BC9WPLANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22834646
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC369200.16168413558940217No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC355290.15559251498797047No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG279190.12226596374649294No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA271580.11893330862234519No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC269110.11785161898283862No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC255700.11197896389547708No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC253080.11083158460174947No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC247890.10855872256570126No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG233640.10231820541470185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCA83950.018.2531974
CGGTGGC90200.018.1326311
GGCCTTA92750.015.8339544
CCTTATA95650.015.378396
CGATACG16650.015.0542926
GCCTTAT97800.014.9041835
GTGGCGC110100.014.2358473
GCGCACG110450.013.9945636
CGCACGC123250.012.6468497
GAGGTAA119050.012.50023421
GATCTCG23100.012.4749234
GGTAAAA119500.012.32737823
ATTACAC117900.012.325229
GCACGCC128450.012.2336998
GCGATCT17150.012.1770083
AACGATA21750.012.1296664
AATTAGA120650.012.12317916
ATCTCGG23850.011.98981535
AGAGGTA121200.011.98658420
GAACGAT22550.011.8930813
Produced by FastQC (version 0.11.2)