FastQCFastQC Report
Mon 31 Oct 2016
G142-M10-Index-22_CGTACG_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M10-Index-22_CGTACG_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22328103
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA712050.3189030433978202No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC655850.29373296961233114No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC558380.2500794626395265No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG533370.2388783319389023No Hit
GACGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGAC510850.22879238778144295No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA484140.21682988474211176No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG375820.16831703078403032No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG312200.13982379067312617No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG311000.13928635137521536No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG296080.13260418943785776No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAG295320.13226381121584757No Hit
CGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGG279850.12533532293361418No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT268340.12018038433448647No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT268300.12016246969122277TruSeq Adapter, Index 22 (97% over 40bp)
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC267740.11991166468553105No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT257290.11523146413289119No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG253970.11374454874200464No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC252140.11292495381269067No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG247480.1108378978724704No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG246260.11029150125292776No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC241760.10827610388576227No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT240820.10785510976906547No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGG236300.10583075508026814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC39000.034.74917232
TACGTAA39450.034.01484336
CCGTACG39950.033.97797833
CGTACGT42000.032.36979734
CACCGTA42900.031.89749731
AATCTCG39350.031.08756641
CGTAATC39050.030.81973838
ACGTAAT41550.030.65676937
ATCTCGT42800.028.47987442
GTCACCG49200.028.3041429
TCACCGT55000.025.39921430
GATCGGA57700.025.1760031
TCTCGTA53750.024.3936643
ATCGGAA60450.023.9927162
ACGTCTG61100.023.91478215
CGTCTGA62800.023.19711116
GTACGTA61100.022.10599935
CTCGTAT59950.021.94385144
GTAATCT59700.020.71190539
GCACACG72500.020.12380611
Produced by FastQC (version 0.11.2)