FastQCFastQC Report
Mon 31 Oct 2016
G142-M11-Index-23_GAGTGG_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M11-Index-23_GAGTGG_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9849514
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC181220.18398877345623346No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC114190.11593465423776238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG15450.026.89980915
TCGTGTA15200.026.18036814
AGCGTCG15450.026.0179310
GATCTCG15350.025.80006634
CGTCGTG15850.025.6447512
ATCTCGG16000.024.74161535
GATCGGA17450.023.8007581
GAGCGTC18850.021.3410859
AGAGCGT18750.021.3385318
TGGTCGC18300.020.81819242
GTAGATC19700.020.22270431
TCTCGGT20500.019.418536
GTGGTCG19400.019.41045641
AAGAGCG20500.019.4099127
GTCGTGT22300.018.72328413
ATCGGAA23500.017.5802672
TAGATCT23000.017.50589632
TGTAGGG25250.017.16823417
GTGTAGG25650.016.9777416
GTAGGGA24200.016.54860318
Produced by FastQC (version 0.11.2)