FastQCFastQC Report
Mon 31 Oct 2016
G142-M12-Index-25_ACTGAT_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M12-Index-25_ACTGAT_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15772452
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG516510.32747603226181954No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT437660.2774838053081411No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG416180.26386512382475474No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC365060.23145418353468442No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA338450.21458299571937198No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGA326780.20718401932686178No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC290850.1844037946668026No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC263960.16735508213941624No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG239850.15206893639619254No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGC236090.1496850331197711No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC207780.13173601669543836No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG207210.13137462710300213No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT204630.12973886368460655TruSeq Adapter, Index 25 (97% over 44bp)
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG196240.12441946249067677No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGACGCGTG180350.11434493508048083No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC178560.11321004495686529No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG172000.10905089455970447No Hit
GACGGGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGAC169960.10775750022888007No Hit
GGGGAACGACGCGCGGGTGGAGGGGTCGGGAGGAACGGGGAGCGGGAAAG169890.10771311905086159No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT166390.10549406014993737No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT165990.10524045341840317No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG163520.10367443185117951No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC158470.10047264686556029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG25600.035.77540641
ATCTCGT32300.028.62702842
TCTCGTA36300.026.74271443
GATCGGA42650.025.3236871
CGTCTGA43250.025.15688116
ACGTCTG43900.024.784415
ATCGGAA45350.023.8144742
CTCGTAT41950.023.35001844
ACACGTC50850.021.48375913
ATATCTC43800.021.4112640
ACTGATA49550.021.41054334
GATATAT45750.021.26634437
TGATATA49200.021.2041636
CTGATAT49650.021.18905435
GCACACG54600.020.04877311
TCGGAAG54650.019.9635263
GTCGGAT16350.019.952341
CACACGT56300.019.56099512
CACGTCT56700.019.42287414
GTCACAC56300.019.0005829
Produced by FastQC (version 0.11.2)