FastQCFastQC Report
Mon 31 Oct 2016
G142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M13-Index-27_ATTCCT_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16298935
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT246520.15124914603316106No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG238750.14648196339208666No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG230220.14124849261623534No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG216460.13280622322869562No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC211710.12989192238633998No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT201500.12362770941782393No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA192500.11810587624283427No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA177780.10907461131662897No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT163670.10041760397228408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGT32550.016.452832
ATCGCCG22150.015.1045827
ACATCGC21600.015.052475
TATCCGA75050.014.339869538
TTATCCG76200.014.15227837
GCCGACA24000.013.9586811
CGCCTAT5850.013.9398431
CGACATC23500.013.5982013
GTACGTA23200.013.3950141
TATCTCG7450.013.26681741
CCGACAT24300.013.059952
ATCCGAG83950.012.92486739
ACCCGAC85350.012.892961543
GTCCGCG87050.012.8872731
GCCGGGT16900.012.7806051
GAGTCGC24550.012.5259616
TCCGCGG87900.012.486072
GCGGGTA21150.012.4007691
GTCGGTT16000.012.3975121
GTCGGCT16250.012.3424131
Produced by FastQC (version 0.11.2)