FastQCFastQC Report
Mon 31 Oct 2016
G142-M15-Index-1_ATCACG_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M15-Index-1_ATCACG_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17641715
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1222050.6927047625471787Illumina Single End PCR Primer 1 (100% over 50bp)
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC574370.32557492284621986No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC362170.20529183245506458No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC361570.20495172946621118No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC338550.19190311146053543No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC283190.160522942355661No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC282740.16026786511402094No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC264320.14982670335622134No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC251530.14257684131049617No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT251300.14244646849810236No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA250460.1419703243137076No Hit
CCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGG192090.10888397188141856No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG186670.10581170821544277No Hit
GCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGC177370.10054011188821495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC162650.038.9645342
GTGGTCG169400.038.2822341
GATCTCG178500.037.48460834
ATCTCGG179900.037.3837235
TCTCGGT188250.035.86602836
GGTCGCC177450.035.8140243
TCGGTGG193000.035.09932738
TAGATCT197050.034.30259332
CTCGGTG196900.034.1917237
GTAGATC199300.033.89367331
TCGTGTA212500.031.85772114
AGATCTC209700.031.84454533
CGTGTAG213050.031.84282515
GAGTGTA212200.031.78733427
CGTCGTG218050.031.47683512
TGTAGAT216100.031.3419530
AGTGTAG217400.030.95469528
AGCGTCG219300.030.83775510
GTAGGGA217150.030.68466818
TGTAGGG226450.030.39623517
Produced by FastQC (version 0.11.2)