FastQCFastQC Report
Mon 31 Oct 2016
G142-M8-Index-20_GTGGCC_BC9WPLANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M8-Index-20_GTGGCC_BC9WPLANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14633071
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT821940.5617002746723501TruSeq Adapter, Index 20 (97% over 44bp)
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC485770.3319672268384401No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG430900.29446997147762083No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGC399620.27309373404940085No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACG355260.24277883979377946No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA347030.23715459318143128No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGG334330.22847562210283814No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG283350.1936367287495564No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC262170.17916266517124121No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC261250.17853395230570535No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGG213270.14574520960091017No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAA210870.1441050890821209No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGG204680.13987494491074362No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGC198600.13571997292981083No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGG186490.12744419814542005No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCC183340.12529153996450915No Hit
GGAAGAAGGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGG175790.12013199416581796No Hit
CCCGTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGG173540.11859438117945303No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA171570.11724811558694685No Hit
GTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTT168280.11499978370910657No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC167120.11420705879169178No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCC166890.11404988057530782No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT155440.10622513893358407No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTCGTCGGGAGAC146940.10041637876287213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG92250.040.83451541
GTCACGT107100.039.34057629
ACGTGGC107300.038.95975532
AGTCACG108000.038.935928
GATCGGA109900.038.7336771
TCACGTG108550.038.69377530
CGTGGCC109700.037.947233
ACGTCTG115650.037.2808115
CGTCTGA116200.037.08536516
CTCGTAT109350.036.65841744
TCTCGTA105350.036.61192743
CACGTGG115050.036.4504131
ATCGGAA119600.035.681582
CCTTATC106400.035.567838
TCGGAAG122000.035.1420143
ACACGTC124750.034.68514313
CGGAAGA124400.034.6217274
GGCCTTA120350.034.32976536
GCACACG126800.034.00175511
CACACGT128350.033.67709412
Produced by FastQC (version 0.11.2)