FastQCFastQC Report
Mon 31 Oct 2016
G142-M8-Index-20_GTGGCC_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M8-Index-20_GTGGCC_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14633071
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG667680.45628152832717067Illumina Single End PCR Primer 1 (100% over 50bp)
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC402620.27514388469788736No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC319620.218423050089759No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC299440.20463237006093934No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC285970.1954271936492347No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC279640.191101375780928No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG271500.18553863368803444No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA265280.1812879880101723No Hit
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT261490.17869796435758428No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC248130.16956796013632408No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT245080.16748364031036272No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC232670.1590028504611233No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC216600.14802087682073026No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG199270.13617783990797283No Hit
CCGGCCGTCGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCG199220.13614367073049805No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC195910.13388167118166788No Hit
GCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCC184070.1257904099556409No Hit
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCG179320.12254433809553715No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG169980.11616153574324899No Hit
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC169250.11566266575211726No Hit
GCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGG164140.11217057581419511No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC159750.10917052203190977No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC158680.10843930163394956No Hit
CTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATAG151620.10361461377451117No Hit
CGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGG147100.10052572013079142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG91900.039.624634
TCGTGTA94300.039.40322514
CGTGTAG95900.039.20864515
ATCTCGG93950.038.67025435
CGTCGTG99150.038.2695512
GATCGGA102550.037.9290121
GTAGATC97800.037.697831
AGAGCGT101050.037.4609958
GTGGTCG95150.037.3075941
TAGATCT100650.036.90149732
TCTCGGT100150.036.73730536
AAGAGCG102550.036.7211347
GTCGTGT105000.036.24783713
GAGTGTA105250.035.6746427
TGTAGGG108150.035.54487617
GTAGGGA104150.035.5169318
GTGTAGG109300.035.253216
ATCGGAA109450.035.1970332
TCGGTGG106400.034.8833938
GGTCGCC100800.034.6270643
Produced by FastQC (version 0.11.2)