FastQCFastQC Report
Mon 31 Oct 2016
G142-M9-Index-21_GTTTCG_BC9WPLANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG142-M9-Index-21_GTTTCG_BC9WPLANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19681557
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC695630.3534425655449922No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG637880.32410037478234066Illumina Single End PCR Primer 1 (100% over 50bp)
GGACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTT511150.2597101438671747No Hit
CGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCC498260.25316086527097426No Hit
GGCGCCGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCC476730.24222169008275107No Hit
CGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGC451970.22964138457135275No Hit
CGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTG384580.1954012073333426No Hit
CCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCG378020.1920681376986587No Hit
CCTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATA374160.1901069107489819No Hit
CGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGT362560.1842130681022848No Hit
CGCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGC360150.18298857148344513No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC357380.18158116250660455No Hit
GCCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCC335290.17035745698371324No Hit
GTCGCCTCTCTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGC334660.17003736035721156No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC304400.1546625604874655No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC298890.15186298523028438No Hit
GTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG275680.1400702190380568No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTC272490.1384494123102151No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG264080.13417637639135968No Hit
GCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC258670.13142761012251217No Hit
GCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCC242650.12328801019147011No Hit
CGCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGG239330.12160115177879474No Hit
GGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGG217110.11031139457106977No Hit
GCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCCGTC215530.10950861255539894No Hit
GCCGGCAGTCGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGG208260.10581479910354652No Hit
CTGGCCTTATAATTAATTAGAGGTAAAATTACACATGCAAACCTCCATAG204300.10380276316553615No Hit
GGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGG199740.10148587329752418No Hit
CTCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGC198640.10092697442585462No Hit
CGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCCGTCCC197810.10050525982268578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGG92000.035.9294335
GATCTCG92950.035.72017734
TGGTCGC94150.034.3152742
GTGGTCG96600.033.80912441
GTAGATC102100.033.11443331
TCGTGTA104950.032.4697714
TAGATCT105000.032.33332432
CGTGTAG105650.032.28037315
TCTCGGT104050.031.95851936
CGTCGTG109200.031.56352812
TCGGTGG108150.031.2273938
GGTCGCC104650.031.0406243
CTCGGTG110700.029.92216337
AGAGCGT115050.029.9001738
GTAGGGA113550.029.84658818
GAGTGTA113600.029.71873727
AAGAGCG114450.029.6743957
GTCGTGT118150.029.4188313
GATCGGA117950.029.2872031
AGCGTCG117600.029.28301610
Produced by FastQC (version 0.11.2)