FastQCFastQC Report
Sat 13 Feb 2021
TC8_A1_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A1_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14141189
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4047902.8624891443003837No Hit
GCCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTC967790.6843766814798954No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA739000.5225868913851586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC705600.49896794392607297TruSeq Adapter, Index 1 (100% over 50bp)
GTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACT667900.4723082337701589No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC646950.4574933550495648No Hit
CAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCT530790.3753503329882657No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT512720.36257205812043103No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC508690.35972222703479884No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT494680.3498149978760626No Hit
CCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCC430460.30440156057598833No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC412700.29184250348397156No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC391560.2768932654814245No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC369940.2616045935034175No Hit
CTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGG341840.2417335628567018No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGCC330290.23356593282219765No Hit
GCAGAAGGTAGGGAGAGGGGCTCTAGCGGTGGCCAGTGGAGCAGCTGGGG305850.21628308623836368No Hit
GGCTGAGTAAGCATTAGACTGTAAATCTAAACACAGAGGTTTAAATCCTC302490.2139070484101443No Hit
GCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGC279240.1974657152238047No Hit
ATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGC266490.18844950025065077No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA254420.17991415007606504No Hit
TGTAAATCTAAACACAGAGGTTTAAATCCTCTTTTTACCAGAGGTCTTAA249570.17648445261568882No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG236080.16694494359703416No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG235490.1665277226688647No Hit
GTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACTAAGACTTCTAC229880.16256058808067694No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG225300.15932182223149693No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA223470.158027730200056No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA214560.15172698703058138No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA213830.15121076452623608No Hit
GGCAGAAGGTAGGGAGAGGGGCTCTAGCGGTGGCCAGTGGAGCAGCTGGG205730.14548281619035003No Hit
CCCGACTCCGGGAAGACCCGAGCCCGGCGCGCCGGGGGCCGCTACCGGCC203560.14394829175962504No Hit
CTTAAGGTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATC201480.14247741119929874No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC199930.14138132232020942TruSeq Adapter, Index 1 (100% over 50bp)
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA198410.14030644806458636No Hit
CCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAG192570.1361766680298241No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA191270.13525736767962016No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT190150.13446535507021368No Hit
AGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGA187040.13226610577087966No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG178800.12643915585881782No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC177460.12549156934399222No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC177150.12527235156817437No Hit
GTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCTGC175270.12394290183095637No Hit
GCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC172410.1219204410605077No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG165880.11730272468602182No Hit
GCTGAGTAAGCATTAGACTGTAAATCTAAACACAGAGGTTTAAATCCTCT163790.1158247725845401No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGC163790.1158247725845401No Hit
CAGAGGTCTTAAGGTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAG163000.11526612083326232No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG153400.10847744132406405No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA152260.10767128563234675No Hit
TTTTTTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCAAT151820.10736013782150851No Hit
CGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCA151050.10681562915254156No Hit
CTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC148750.10518917468679614No Hit
TTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACTAAGACT146950.10391629727882147No Hit
CATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACT146600.10366879333838193No Hit
TTTTTTTTTATTGAGAAAGACATATAGGATATGAGATTGGCTTGAAACCA146390.10352029097411825No Hit
TGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACTA141470.1000410927256541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)