FastQCFastQC Report
Sat 13 Feb 2021
TC8_A1_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A1_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14141189
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3666002.5924269875750903No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1837361.299296685731306No Hit
CTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAG1674871.1843912134969696No Hit
AAGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGC782730.5535107408577878No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG697870.4935016426129373Illumina Single End PCR Primer 1 (100% over 50bp)
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACT583420.41256785408921415No Hit
CCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCA545200.3855404237932185No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC475400.3361810665284228No Hit
ACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAA370660.26211374446660746No Hit
TGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTGGCAGGA357560.25285002555301395No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC339480.2400646791440239No Hit
GGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGG331540.2344498754666245No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA322660.22817034692061608No Hit
CCTATGTAAAAGTAAAATAAGAAAGGAAGGAATCGAACCCCCTAAAATTG287830.2035401690763061No Hit
AGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCT258790.18300441356098135No Hit
ATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTG256800.18159717687105378No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC244190.17267996347407563No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC237140.16769452695950815No Hit
GGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGC231690.16384053702980703No Hit
CGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTG224380.15867124044519876No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCC221410.15657099272204056No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC217070.15350194386059052No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT207970.14706684140916298Illumina Single End PCR Primer 1 (100% over 50bp)
GGCGTACGGAAGACCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC203400.14383514710113837No Hit
CTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGAT202560.14324113764408353No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACTC191270.13525736767962016No Hit
GCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTGGCAGGAA187920.13288840139255617No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATG186510.1318913140896427No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG183770.129953711813059No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG181640.12844747354695565No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG176400.12474198598151824No Hit
AATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTGGCAGGAATTA173480.1226770959641371No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG169320.11973533484348452No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG167990.11879481986981433No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG159300.11264965060575882No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG154950.10957353020315334No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT150110.10615090428393256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)