FastQCFastQC Report
Mon 8 Mar 2021
TC8_A2_mCTRL_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A2_mCTRL_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20444331
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1556720.7614433556177505No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG464320.22711430371578312No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA307610.15046224794540844No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACT287890.14081654224831322No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCT269870.13200236290441592No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC221120.10815712189359485No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT213790.10457177591186526Illumina Single End PCR Primer 1 (100% over 50bp)
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC212910.10414133874079813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)