FastQCFastQC Report
Mon 8 Mar 2021
TC8_A3_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A3_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13555450
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1015920.7494550162480773TruSeq Adapter, Index 8 (100% over 50bp)
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCG327590.24166663592872242No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC264470.19510233891165546TruSeq Adapter, Index 8 (100% over 50bp)
GGGGAGTGGAAGCGGGCACCTCACTGGGCAGTCAGAGTCTCGCTGGCCTT215290.15882172852985332No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCG212760.15695532055372563No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC212630.1568594181676005No Hit
GTGGGAAGACTGGGCCTGGCACATCTCACTTAGCCGCAGGAGCCGGTTTC193700.1428945553264554No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA183720.13553220291469484No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG182340.13451416220044338No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG176600.1302797029976873No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG168040.12396489972667821No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGA166760.12302063007867685No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCT156540.115481227107916No Hit
GCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCC156300.11530417654891575No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA155820.11495007543091523No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC155460.11468449959241486No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT148260.10937298282240723No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC143840.10611230169415255No Hit
GGATGCTGTATGGATAGGAAGGACTGATCCTGGAGTCCTGGTGAGAAGTC143760.10605328484115245No Hit
GGGGTGGGAAGACTGGGCCTGGCACATCTCACTTAGCCGCAGGAGCCGGT138690.10231309178227208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)