FastQCFastQC Report
Mon 8 Mar 2021
TC8_A7_mCTRL_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_A7_mCTRL_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28547265
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3247361.1375380443625687TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1453650.5092081500627118TruSeq Adapter, Index 2 (100% over 50bp)
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT1179800.4132795208227479No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1028340.3602236501465202No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCT1015510.35572934920385546No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGA894530.3133505083586816No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT838670.2937829595935022No Hit
ATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTG837800.29347820185226153No Hit
CCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCT595260.20851734833442012No Hit
CTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCTCTT590770.2069445181526146No Hit
GTCCTCCATCAGTGCCGTCAGTTCTTGTGTGACTTGGGAGCTCTGCAGCT522480.18302278694649032No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC416810.14600698175464444No Hit
CTCTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTC405270.1419645629800263No Hit
CTCTCTTGTCAGAGGCCAGGATAATAGTATGCCATTCCCCATTAATCTTT400180.14018155504564098No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCTTCT393310.13777501977860226No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT362450.1269648773709145No Hit
CGTAGAACTAGCTTCTTCTGCATGGACACAGACTAGGGTCAGTCCCAAAC344890.12081367514541236No Hit
CCCCATTAATCTTTTCTACATTAAAGTTCCTTTCCATAGAACTAGCTTCT337750.11831255988971273No Hit
GTTGAGGGTTTGGAGGAGGTGTTGGAAGGACTTGTGGATGTCAGCCTCCG332370.11642796604158052No Hit
AATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCAT323110.1131842227267656No Hit
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG296090.10371921793558858No Hit
GCCGCCTTTCCACCAGGGATCCACTACAGCACTTGGTAACATGCTCACTC295560.10353356092080974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)