FastQCFastQC Report
Mon 8 Mar 2021
TC8_B1_mArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B1_mArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730294
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTC263310.3912310517192859No Hit
GTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACT253810.37711576938540875No Hit
GCAGAAGGTAGGGAGAGGGGCTCTAGCGGTGGCCAGTGGAGCAGCTGGGG186030.2764069444811772No Hit
GGCAGAAGGTAGGGAGAGGGGCTCTAGCGGTGGCCAGTGGAGCAGCTGGG163460.24287200529427094No Hit
CAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCT145410.2160529688599042No Hit
CCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCC134600.1999912633831449No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC128230.19052659512348197No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT125630.18666346522157873No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC113770.16904164959212778No Hit
GGCTGAGTAAGCATTAGACTGTAAATCTAAACACAGAGGTTTAAATCCTC101810.15127125204337286No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT98030.14565485549368273No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC97430.1447633639778589No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC95100.14130140525807638No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG89640.1331888324640796No Hit
CTTAAGGTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATC89190.13252021382721169No Hit
ATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTTTCTT84980.12626491502451453No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG84030.1248533867911268No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC80420.11948957950425344No Hit
GTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACTAAGACTTCTAC71690.1065183779490168No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCA70480.10472053672543874No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC69860.10379932882575411No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA69330.10301184465344307No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTTTC68890.1023580842085056No Hit
CAGAGGTCTTAAGGTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAG67670.10054538479299716No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGCC67540.100352228297902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)