FastQCFastQC Report
Mon 8 Mar 2021
TC8_B1_mArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B1_mArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730294
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAG691191.026983368037117No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT407580.6055901866991249No Hit
AAGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGC287270.42683127958451744No Hit
CCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCA229220.34057947542856226No Hit
ACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAA125180.18599484658471085No Hit
AGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCT106880.15880435535208418No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC103450.15370799551995795No Hit
TGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTGGCAGGA102710.15260848931710858No Hit
ATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGATTG98560.1464423396659938No Hit
CTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCAACTAGAT79920.11874666990773361No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA77490.11513612926864711No Hit
CTGAGAAGTCCAATTCCAGTCTATCCACATGTTACCTAGGATACCTCATC76100.11307084059032191No Hit
CTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAACCTATGT71760.10662238529252957No Hit
GGAGAAACCGGCTCCTGCGGCTAAGTGAGATGTGCCAGGCCCAGTCTTCC71190.10577546835249695No Hit
CCACATGTTACCTAGGATACCTCATCAAGAATCAAAGACTTCTTTAAATT70100.10415592543208366No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACT68980.10249180793587917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)