FastQCFastQC Report
Mon 8 Mar 2021
TC8_B2_mArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B2_mArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16936155
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1431080.8449851811110609TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC514580.30383519754040983TruSeq Adapter, Index 2 (100% over 50bp)
GTGATATTCATGTCGAATTGCAAATTCGAAGGTGTAGAGAAATCTCTACT353260.20858335318730847No Hit
GCCAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTC329090.19431210921251016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192660.11375663484421346No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC190560.11251668398169479No Hit
CAGTAATAGGGTATTTAGCTGTTAACTAAATTTTCGTAGGTTTAATTCCT179410.10593313535451228No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC177970.10508288333449947No Hit
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTTTC173180.10225461446237354No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAGTCATTCTAAATTTG170460.10064858286901603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)