FastQCFastQC Report
Mon 8 Mar 2021
TC8_B2_mArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B2_mArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16936155
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1418340.8374628125451142Illumina Single End PCR Primer 1 (100% over 50bp)
CTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAG797030.47060858854917187No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG517820.3057482645854387Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT516950.3052345706566809No Hit
AAGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGC399380.2358150359393853No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT302800.17878910531935968No Hit
CCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAGACCTCA268300.15841848400655284No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT215590.12729571735733405No Hit
CGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAGACTTCTCAC212510.12547712275897333No Hit
GAAACCTTCCAGCTGATGGAGCTCTATGGCCGAGAACCAGATTTGAGTTC200300.11826769417261475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187040.1104382901549968No Hit
CTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAATTCCAGTCTA179140.10577371310075989No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)