FastQCFastQC Report
Mon 8 Mar 2021
TC8_B3_mArs_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B3_mArs_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17142541
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1563080.9118134820269643No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT678250.39565312983646944Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG580000.3383395728789565Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC435740.2541863542866836No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG398050.23220011549046318No Hit
CTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGCTAAG373510.2178848514931363No Hit
GCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTTTGGTGACT349360.20379709169136592No Hit
AAGACTTCATCCTACATCTATTGAATGCAAATCAATTGCTTTAATTAAGC301390.17581407563791157No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC238280.13899923004413406No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC222980.13007406544922365No Hit
GGAGAAACCGGCTCCTGCGGCTAAGTGAGATGTGCCAGGCCCAGTCTTCC217730.12701150897057792No Hit
GGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATG200240.11680882081600387No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATG199090.11613797511115767No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA178550.10415608747851325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)