FastQCFastQC Report
Mon 8 Mar 2021
TC8_B5_mArs_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTC8_B5_mArs_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36400266
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT1794620.49302386966073275No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC617500.1696416174541142TruSeq Adapter, Index 7 (100% over 50bp)
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC613930.16866085539045236No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGAGC595640.163636166834605No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT527120.1448121285707088No Hit
GGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCC435820.11972989428154178No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC410260.11270796757364356No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC397710.10926019057113483No Hit
TACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGC386000.10604318111301714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)